neuronal markers β iii tubulin  (Novus Biologicals)

Journal: Investigative Ophthalmology & Visual Science

Article Title: A Novel Murine Model for Lupus-Like Ocular Chronic Graft-Versus-Host Disease

doi: 10.1167/iovs.65.6.20

Figure Lengend Snippet: Histological changes of the cornea in the lupus-like cGVHD model. The cornea underwent histological analysis at week 2, week 6, and week 10 following transplantation ( n = 5 mice). ( A ) Immunofluorescence staining of corneal fiber nerves stained with β-III Tubulin in corneal whole mounts (Bar = 500 µm) and higher-magnification images of the central cornea (Bar = 100 µm). ( B ) H&E staining was performed to assess the histological changes in the central cornea (Bar = 50 µm). ( C ) Higher-magnification images of the central cornea were obtained to quantitatively assess the number of corneal endothelial cells ( blue arrows ; Bar = 25 µm). ( D ) Quantification of corneal endothelial cell density in two groups. Data were presented as mean ± SD. ns = not significant. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: After blocking with PBS containing 5% bovine serum albumin (BSA) and 0.3% triton (Sigma), the corneas were incubated with neuron-specific marker β-III tubulin antibody (1:50; ab52623; Abcam, Waltham, MA, USA) at 4°C overnight.

Techniques: Transplantation Assay, Immunofluorescence, Staining

Journal: PLoS ONE

Article Title: Cofactor-Activated Phosphorylation Is Required for Inhibition of Cortical Neuron Differentiation by Groucho/TLE1

doi: 10.1371/journal.pone.0008107

Figure Lengend Snippet: (A) Primary cultures of E13.0 mouse embryonic cortical progenitor cells were transfected with plasmids encoding either GFP alone (Control; top row) or a combination of GFP and wild type Gro/TLE1 (Gro/TLE1; bottom row). After fixation, cells were subjected to double-labeling analysis of the expression of GFP (green) and either Ki67, nestin, NeuN, or type III β-tubulin (βIIItubulin) (red), as indicated. Arrowheads point to examples of double-labeled cells. ( B–E ) Quantitation of the percentage of GFP-positive cells that co-expressed nestin (B), type III β-tubulin (C), Ki67 (D), or NeuN (E) in the absence or presence of wild type or mutated forms of Gro/TLE1. The anti-neurogenic activity of Gro/TLE1 was impaired by the SP mutations S286A, S289A, and S298A. Results are shown as the mean±the standard deviation (>500 cells were counted in each case; n≥5; **, P <0.001 using the Student's t test).

Article Snippet: Three days after transfection, cells were analyzed by immunocytochemistry using antibodies against the proliferating cell marker Ki67 (1∶200; BD Pharmigen), the neural progenitor cell marker nestin (1∶400; Millipore), the neuronal cell marker type III β-tubulin (1∶300; Promega, Madison, WI), and the neuronal cell marker neuron specific nuclear protein (NeuN) (1∶100; Millipore).

Techniques: Transfection, Labeling, Expressing, Quantitation Assay, Activity Assay, Standard Deviation

Journal: Journal of Neuroendocrinology

Article Title: Localisation of oestrogen receptors in stem cells and in stem cell‐derived neurons of the mouse

doi: 10.1111/jne.13220

Figure Lengend Snippet: Details of primary and secondary antibodies used for immunocytochemistry in mouse embryonic stem cell line (mES) and cultured differentiated neurons (mESn). Primary antibodies were bought from various sources and used at the dilution described at the table. Goat anti‐rabbit secondary antibodies conjugated to different fluorophores was used to obtain localisation of different antigens from the same coverslip

Article Snippet: β‐tubulin‐III /neuronal marker (ab 18207) , Abcam, UK. , Mouse , 1:300.

Techniques: Immunocytochemistry, Cell Culture, Marker