neuronal markers β iii tubulin  (Novus Biologicals)

Journal: Investigative Ophthalmology & Visual Science

Article Title: A Novel Murine Model for Lupus-Like Ocular Chronic Graft-Versus-Host Disease

doi: 10.1167/iovs.65.6.20

Figure Lengend Snippet: Histological changes of the cornea in the lupus-like cGVHD model. The cornea underwent histological analysis at week 2, week 6, and week 10 following transplantation ( n = 5 mice). ( A ) Immunofluorescence staining of corneal fiber nerves stained with β-III Tubulin in corneal whole mounts (Bar = 500 µm) and higher-magnification images of the central cornea (Bar = 100 µm). ( B ) H&E staining was performed to assess the histological changes in the central cornea (Bar = 50 µm). ( C ) Higher-magnification images of the central cornea were obtained to quantitatively assess the number of corneal endothelial cells ( blue arrows ; Bar = 25 µm). ( D ) Quantification of corneal endothelial cell density in two groups. Data were presented as mean ± SD. ns = not significant. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: After blocking with PBS containing 5% bovine serum albumin (BSA) and 0.3% triton (Sigma), the corneas were incubated with neuron-specific marker β-III tubulin antibody (1:50; ab52623; Abcam, Waltham, MA, USA) at 4°C overnight.

Techniques: Transplantation Assay, Immunofluorescence, Staining

Journal: PLoS ONE

Article Title: Cofactor-Activated Phosphorylation Is Required for Inhibition of Cortical Neuron Differentiation by Groucho/TLE1

doi: 10.1371/journal.pone.0008107

Figure Lengend Snippet: (A) Primary cultures of E13.0 mouse embryonic cortical progenitor cells were transfected with plasmids encoding either GFP alone (Control; top row) or a combination of GFP and wild type Gro/TLE1 (Gro/TLE1; bottom row). After fixation, cells were subjected to double-labeling analysis of the expression of GFP (green) and either Ki67, nestin, NeuN, or type III β-tubulin (βIIItubulin) (red), as indicated. Arrowheads point to examples of double-labeled cells. ( B–E ) Quantitation of the percentage of GFP-positive cells that co-expressed nestin (B), type III β-tubulin (C), Ki67 (D), or NeuN (E) in the absence or presence of wild type or mutated forms of Gro/TLE1. The anti-neurogenic activity of Gro/TLE1 was impaired by the SP mutations S286A, S289A, and S298A. Results are shown as the mean±the standard deviation (>500 cells were counted in each case; n≥5; **, P <0.001 using the Student's t test).

Article Snippet: Three days after transfection, cells were analyzed by immunocytochemistry using antibodies against the proliferating cell marker Ki67 (1∶200; BD Pharmigen), the neural progenitor cell marker nestin (1∶400; Millipore), the neuronal cell marker type III β-tubulin (1∶300; Promega, Madison, WI), and the neuronal cell marker neuron specific nuclear protein (NeuN) (1∶100; Millipore).

Techniques: Transfection, Labeling, Expressing, Quantitation Assay, Activity Assay, Standard Deviation